enhanced green fluorescent protein ad. egfp  (Regeneron inc)

Journal: The Journal of Physiology

Article Title: Differential regulation of blood flow‐induced neovascularization and mural cell recruitment by vascular endothelial growth factor and angiopoietin signalling

doi: 10.1113/JP273430

Figure Lengend Snippet: A , images of mesenteries after treatment with Ad. eGFP , and Ad. eNOS /Ad. Ang1 . B , angiogenesis index. C , confocal stack images from mesenteric panels stained with isolectin B4 and Hoechst 33324 or Ki67 and Hoechst 33324. D – I , analysis of vessel density ( D ), branch point density ( E ), proliferating endothelial cell density ( F ), exchange vessel density ( G ), conduit vessel density ( H ) and sprout point density ( I ). * P < 0.05, ** P < 0.01, *** P < 0.001 compared with GFP; # P < 0.05 compared with eNOS. Scale bar: 40 μm.

Article Snippet: Ang1 and Ad. eGFP (enhanced green fluorescent protein) were a gift from Regeneron Inc., Tarrytown, NY, USA (Benest et al . ) and Ad. eNOS (endothelial nitric oxide synthase) from Prof. Keith Channon, University of Oxford (Benest et al . ); Ad. sFlt1 (soluble Fms‐like tyrosine kinase receptor‐1/soluble VEGFR1) was generated by Dr Ewa Paleolog, Imperial College London (Afuwape et al . ) and Ad. sTie2 (soluble Tie2) (Lin et al . ) by Prof. Charles Lin, Vanderbilt University, Nashville, TN, USA.

Techniques: Staining

Journal: The Journal of Physiology

Article Title: Differential regulation of blood flow‐induced neovascularization and mural cell recruitment by vascular endothelial growth factor and angiopoietin signalling

doi: 10.1113/JP273430

Figure Lengend Snippet: A , sample confocal stack images from rat mesentery 6 days post‐treatment stained with isolectin‐B 4 and antibodies for NG2 and αSMA. B , treatment with Ad. eNOS +Ad. Ang1 significantly increased mural cell coverage. C , Ad. eNOS was unable to stimulate arteriolargenesis by itself but did so when together with Ad. Ang1 . Scale bar: 40 μm. *** P < 0.001 vs . eGFP; ### P < 0.001 vs . eNOS.

Article Snippet: Ang1 and Ad. eGFP (enhanced green fluorescent protein) were a gift from Regeneron Inc., Tarrytown, NY, USA (Benest et al . ) and Ad. eNOS (endothelial nitric oxide synthase) from Prof. Keith Channon, University of Oxford (Benest et al . ); Ad. sFlt1 (soluble Fms‐like tyrosine kinase receptor‐1/soluble VEGFR1) was generated by Dr Ewa Paleolog, Imperial College London (Afuwape et al . ) and Ad. sTie2 (soluble Tie2) (Lin et al . ) by Prof. Charles Lin, Vanderbilt University, Nashville, TN, USA.

Techniques: Staining

Journal: The Journal of Physiology

Article Title: Differential regulation of blood flow‐induced neovascularization and mural cell recruitment by vascular endothelial growth factor and angiopoietin signalling

doi: 10.1113/JP273430

Figure Lengend Snippet: A , images of mesenteries after treatment with eNOS with dual VEGF–Ang1 inhibition or eNOS and Ang1 (NO–Tie) with VEGF blockade. B , angiogenesis index. C , sample confocal stack images from rat mesentery 6 days post‐treatment stained with isolectin‐B 4 , Hoechst 33324 and antibodies for Ki67. D – F , quantification of vessel density ( D ), branch point density ( E ) and sprout point density ( F ). G , images of mesenteries after treatment with eNOS with VEGF inhibition, angiopoietin inhibition, dual VEGF–Ang1 or eNOS and Ang1 (NO–Tie) with VEGF blockade stained with isolectin‐B 4 and antibodies for NG2 and αSMA. No arteriolargenesis was seen in any of these conditions. # P < 0.05, ### P < 0.001 vs . eGFP; * P < 0.05, ** P < 0.01 vs . eNOS; + P < 0.05 vs . eNOS–sFlt1–sTie2.

Article Snippet: Ang1 and Ad. eGFP (enhanced green fluorescent protein) were a gift from Regeneron Inc., Tarrytown, NY, USA (Benest et al . ) and Ad. eNOS (endothelial nitric oxide synthase) from Prof. Keith Channon, University of Oxford (Benest et al . ); Ad. sFlt1 (soluble Fms‐like tyrosine kinase receptor‐1/soluble VEGFR1) was generated by Dr Ewa Paleolog, Imperial College London (Afuwape et al . ) and Ad. sTie2 (soluble Tie2) (Lin et al . ) by Prof. Charles Lin, Vanderbilt University, Nashville, TN, USA.

Techniques: Inhibition, Staining